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Bio 420/642
 Molecular Biology
Department of Biological Sciences, Lehman College

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Laboratory instructor:

 

 

This is an organizational scheme. 

Return to it frequently to see where you are in the progression of the course.

The first series of exercises are mostly independent from each other and are designed to instill skills in basic wet laboratory and molecular biology techniques. The emphasis includes practice in the presentation of results. No formal reports are required, but great attention should be applied to keeping detailed and accurate notes in a formal notebook and presenting the results from each session in a formal manner. Data should be tabulated, graphs and figures should be completely labeled, as if the results were being submitted for publication. All work must be typed. At the beginning of each lab (except the first) the instructor will check your notebook, and ask you to submit last session's results (just the results). These efforts will be returned with commentary. ^up By the time you start the second series of exercises you will have skill in manipulations in the lab and in collecting and presenting results. This series consists of four sets of sessions where the results of one session are dependent on the success of the previous session. You will present formal reports on each of these four sets of sessions after all the experiments are completed. Due dates will be assigned. The flow through each of these two series of exercises and the relationship among sessons within a sets is given below.

 

Also see Overview.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Experiment 

Experiment Series 1

Basic Techniques , Metric System, Solution Calculations, Notebook Keeping
Molecular Biology on the Internet
Restriction Digestion and Gel Electrophresis
Transformation and Serial Dilution

 

Experiment Series 2

Set 1

Set 2

Set 3

Set 4

Plasmid DNA
preparation
Phage Titering SDS-PAGE PCR
Gel Electrophresis Phage DNA Western Blotting Gel Electrophresis
Restriction Digestion Gel Electrophresis  Western Immunodetection

DIG Detection

Southern Blotting
Southern
Hybridization

Each set forms the basis of one final report. Four final reports will be due. See Overview for more details.

 

Schedule Accessories Internet Experiments Reports Results Checklist Grading Form Report Format Flow Chart