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| Plasmid extraction |
Gel photograph, molecular weight standard
curve, and size of unknown plasmid bands, restriction map of plasmid and insert (supplied) |
| Southern transfer |
none |
| DNA probe hybridization |
Photocopy of probed blot, size of detected
band(s), size standards, standard curve |
| Lambda plating |
Titers from four plates and
calculated stock titer. |
| Lambda DNA extraction |
Gel photograph or photocopy, size standards,
standard curve |
| SDS PAGE electrophoresis (maize proteins) |
Photocopy of gel, size standards, standard
curve, major band sizes |
| Western detection |
Photocopy of blot, size standards, standard
curve of markers, size of antigen |
| Polymerase chain reaction |
Gel photograph or photocopy,
standard curve of markers, size of PCR products, data matrix of scored RAPDs, genetic
distance analysis, cladogram |
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